Isolation and crystallization of ribosomal protein TS9

Human recombinant laminin-binding protein: isolation, purification, and crystallization.

The mRNA of the precursor of laminin-binding protein (LBP) was isolated from a human embryo kidney cell line and cloned. The determined sequence of the LBP gene showed complete identity with the LBP genes isolated from human lung and large intestine cells. The human LBP was expressed by E. coli cells, and it was purified using Ni-NTA-Sepharose chromatography. The mobility of the homogeneous rec...

Isolation of a cDNA encoding human 40S ribosomal protein s3.

A cDNA which encodes human 40S ribosomal protein s3 has been isolated from a lambda-Gem 2 cDNA library constructed using poly (A+) RNA from human WISH cells. The cDNA insert includes a 5' noncoding sequence of 22 nucleotides, an open reading frame of 729 nucleotides and a 3' noncoding sequence of 82 nucleotides followed by a 3' poly A tail. The hexameter AATAAA, which is the signal sequence for...

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

The Isolation and Crystallization of Rabbit

In a previous publication (1) it was found that phosphorylase extracted from excised rabbit skeletal muscle is largely in the form of phosphorylase b, rather than being present mainly as phosphorylase a, as had been reported earlier by Cori (2). This phosphorylase b could be readily converted to phosphorylase a upon addition of divalent metal ions to the crude extracts,’ and it was shown that f...

The Isolation and Crystallization of Human Insulin.